Molecular Vehicles for Targeted Drug Delivery
2002; American Chemical Society; Volume: 13; Issue: 3 Linguagem: Inglês
10.1021/bc0155770
ISSN1520-4812
AutoresMarina V. Backer, Renee Aloise, Kristen Przekop, Konstantin Stoletov, Joseph M. Backer,
Tópico(s)RNA Interference and Gene Delivery
ResumoTargeted drug delivery by cell-specific cytokines and antibodies promises greater drug efficacy and reduced side effects. We describe a novel strategy for assembly of drug delivery vehicles that does not require chemical modification of targeting proteins. The strategy relies on a noncovalent binding of standardized "payload" modules to targeting proteins expressed with a "docking" tag. The payload modules are constructed by linking drug carriers to an adapter protein capable of binding to a docking tag. Using fragments of bovine ribonuclease A as an adapter protein and a docking tag, we have constructed vascular endothelial growth factor (VEGF) based vehicles for gene delivery and for liposome delivery. Assembled vehicles displayed remarkable selectivity in drug delivery to cells overexpressing VEGF receptors. We expect that our strategy can be employed for targeted delivery of many therapeutic or imaging agents by different recombinant targeting proteins.
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