Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Mastoparan induces apoptosis in B16F10-Nex2 melanoma cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivo

2014; Elsevier BV; Volume: 68; Linguagem: Inglês

10.1016/j.peptides.2014.09.024

1873-5169

Ricardo Azevedo, Carlos R. Figueiredo, Adilson Kleber Ferreira, Alisson L. Matsuo, Mariana H. Massaoka, Natália Girola, Aline Vivian Vatti Auada, Camyla F. Farias, Kerly Fernanda Mesquita Pasqualoto, Cecília Pessoa Rodrigues, José Alexandre Marzagão Barbuto, Débora Levy, Sérgio Paulo Bydlowski, Paulo L de Sá-Junior, Luiz R. Travassos, Ivo Lebrun,

Beetle Biology and Toxicology Studies

Mastoparan is an α-helical and amphipathic tetradecapeptide obtained from the venom of the wasp Vespula lewisii. This peptide exhibits a wide variety of biological effects, including antimicrobial activity, increased histamine release from mast cells, induction of a potent mitochondrial permeability transition and tumor cell cytotoxicity. Here, the effects of mastoparan in malignant melanoma were studied using the murine model of B16F10-Nex2 cells. In vitro, mastoparan caused melanoma cell death by the mitochondrial apoptosis pathway, as evidenced by the Annexin V-FITC/PI assay, loss of mitochondrial membrane potential (ΔΨm), generation of reactive oxygen species, DNA degradation and cell death signaling. Most importantly, mastoparan reduced the growth of subcutaneous melanoma in syngeneic mice and increased their survival. The present results show that mastoparan induced caspase-dependent apoptosis in melanoma cells through the intrinsic mitochondrial pathway protecting the mice against tumor development.