Artigo Revisado por pares

Cloning of a human cDNA encoding a putative nucleotide-binding protein related to Escherichia coli MinD

1994; Elsevier BV; Volume: 147; Issue: 2 Linguagem: Inglês

10.1016/0378-1119(94)90082-5

ISSN

1879-0038

Autores

Mandana Shahrestanifar, Deba P. Saha, Louis A. Scala, Amartya Basu, Richard Howells,

Tópico(s)

Ubiquitin and proteasome pathways

Resumo

A novel human cDNA encoding a putative nucleotide-binding protein (NBP) was obtained by screening a human SHSY5Y neuroblastoma library. The deduced protein contains 320 amino acids (aa) with a Mr of 34 540. NBP displays sequence similarity with the product of the minD gene from Escherichia coli. MinD is involved in the proper placement of the division septum, and has ATPase activity. NBP and MinD contain consensus nucleotide (nt)-binding domains. The NBP mRNA is approx. 1500 nt in length and is expressed in several human cell lines and in all rat tissues examined, with the highest levels in lung and testis.

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