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Herpes specific and α DNA polymerase in nuclear envelope of BHK infected cells

1981; Elsevier BV; Volume: 100; Issue: 2 Linguagem: Inglês

10.1016/s0006-291x(81)80224-0

1090-2104

Max J. Herzberg, Viviane Bibor‐Hardy, René Simard,

Virus-based gene therapy research

Nuclear envelopes of normal and Herpes simplex virus infected BHK cells were isolated and separated in two fractions by a step gradient procedure. α, β and Herpes-specific DNA polymerases activity were tested in the chromatin and nuclear envelope fractions of these cells. We found that both α and Herpes-specific DNA polymerases activity were associated with only one of the two nuclear envelope fractions and were released by Triton X-100 treatment. On the contrary, β DNA polymerase activity was associated with the chromatin fraction. More endonucleotylic activity and particularly apurinic and UV specific endonucleases were found associated with the same nuclear envelope fraction. These findings suggest an active role of the nuclear envelope in the replication of both cellular and Herpes simplex viral DNA.