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Functional heterogeneity of human mast cells from different anatomic sites: In vitro responses to morphine sulfate1

1987; Elsevier BV; Volume: 79; Issue: 4 Linguagem: Inglês

10.1016/s0091-6749(87)80162-8

1097-6825

Marla E. Tharp, Anne Kagey‐Sobotka, C. A. FOX, Giancarlo Marone, Lawrence M. Lichtenstein, Timothy J. Sullivan,

Asthma and respiratory diseases

The responses of human cutaneous, pulmonary, intestinal, and cardiac tissue mast cells to the histamine-releasing agent morphine sulfate (MS) were investigated in vitro.Human cutaneous mast cells released significant amounts of histamine in the presence of I to 100 lamol/L concentrations of MS.Histamine release was detectable within 5 minutes after challenge and was complete by 15 minutes.A maximal histamine release of 21.6% (+_-1.4SEM) was observed after stimulation with 100 bunol/L of MS.This MS effect was inhibited by the opiate receptor antagonist naloxone with a 59% inhibition detected at equimolar concentrations and an 88% inhibition occurring in the presence of a tenfold molar excess of naloxone.Naloxone did not alter the cutaneous mast cell response to the calcium ionophore A23187.Human mast cells derived from pulmonary, intestinal, and cardiac tissues, as well as blood basophils, did not release histamine after stimulation with I to 200 Ixmol/L of MS, whereas each of these cell preparations responded to an IgE-mediated stimulus.The results of this study demonstrate that cutaneous mast cells release inflammatory mediators after stimulation by MS, whereas mast cells residing in lung, heart, and gastrointestinal tissues do not.These observations indicate that human mast cells in different anatomic sites can vary in their functional responses.(J ALLERGY CLIN IMMUNOL 1987;79:646-53.)Mast cells derived from different mammalian species vary in their morphologic characteristics and functional responses to some secretory agonists.1-3 Moreover, mast cells from different anatomic sites within the same animal express different morphologic, cytochemical, and functional properties .4-9Experimental observations in rodents indicate that mast cells arising in connective tissues and in the peritoneal cavity have similar properties; however, the phenotypic and functional characteristics of these cells differ from mast