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The Rodent Hippocampus Is Essential for Nonspatial Object Memory

2013; Elsevier BV; Volume: 23; Issue: 17 Linguagem: Inglês

10.1016/j.cub.2013.07.002

1879-0445

Sarah J. Cohen, Alcira H. Munchow, Lisa M. Rios, Gongliang Zhang, Herborg N. Ásgeirsdóttir, Robert W. Stackman,

Zebrafish Biomedical Research Applications

Elucidating the role of the rodent hippocampus in object recognition memory is critical for establishing the appropriateness of rodents as models of human memory and for their use in the development of memory disorder treatments. In mammals, spatial memory [1Eichenbaum H. Yonelinas A.P. Ranganath C. The medial temporal lobe and recognition memory.Annu. Rev. Neurosci. 2007; 30: 123-152Crossref PubMed Scopus (1851) Google Scholar, 2Squire L.R. Stark C.E. Clark R.E. The medial temporal lobe.Annu. Rev. Neurosci. 2004; 27: 279-306Crossref PubMed Scopus (2015) Google Scholar, 3Morris R.G.M. Garrud P. Rawlins J.N.P. O’Keefe J. Place navigation impaired in rats with hippocampal lesions.Nature. 1982; 297: 681-683Crossref PubMed Scopus (5077) Google Scholar, 4Riedel G. Micheau J. Lam A.G. Roloff E.L. Martin S.J. Bridge H. de Hoz L. Poeschel B. McCulloch J. Morris R.G. Reversible neural inactivation reveals hippocampal participation in several memory processes.Nat. 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Transient inactivation of perirhinal cortex disrupts encoding, retrieval, and consolidation of object recognition memory.J. Neurosci. 2005; 25: 52-61Crossref PubMed Scopus (229) Google Scholar, 24Good M.A. Barnes P. Staal V. McGregor A. Honey R.C. Context- but not familiarity-dependent forms of object recognition are impaired following excitotoxic hippocampal lesions in rats.Behav. Neurosci. 2007; 121: 218-223Crossref PubMed Scopus (95) Google Scholar]. In the current studies, we used intrahippocampal muscimol microinfusions to transiently inactivate the male C57BL/6J mouse hippocampus at distinct stages during the novel object recognition (NOR) task: during object memory encoding and consolidation, just consolidation, and/or retrieval. We also assessed the effect of temporary hippocampal inactivation when objects were presented in different contexts, thus eliminating the spatial or contextual components of the task. Lastly, we assessed extracellular dorsal hippocampal glutamate efflux and firing properties of hippocampal neurons while mice performed the NOR task. Our results reveal a clear and compelling role of the rodent hippocampus in nonspatial object memory.