Enzymatic synthesis of 1-O-phenylcarboxyl-β-d-glucose esters
1988; Elsevier BV; Volume: 16; Issue: 1 Linguagem: Inglês
10.1016/0045-2068(88)90035-1
ISSN1090-2120
AutoresSabina Weisemann, Klaus Denzel, Gerhard Schilling, Georg G. Gross,
Tópico(s)Biofuel production and bioconversion
ResumoA procedure for the facile preparation of various 1-O-phenylcarboxyl-β-d-glucose esters has been developed. Although such compounds are widely distributed in higher plants, only fragmentary knowledge exists about their synthesis and properties. The method reported here utilizes a UDP-glucose-specific glucosyltransferase from oak leaves. Under the catalysis of this enzyme, several differently ring-substituted 1-O-benzoylglucoses were synthesized. The reaction products were purified by reversed-phase HPLC and characterized by uv, ir, and 1H NMR spectroscopy. The described procedure appears most suitable for small-scale preparations sufficient for many biochemical investigations, and particularly for the synthesis of radioactively labeled esters.
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