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Sample processing obscures cancer-specific alterations in leukemic transcriptomes

2014; National Academy of Sciences; Volume: 111; Issue: 47 Linguagem: Inglês

10.1073/pnas.1413374111

1091-6490

Heidi Dvinge, Rhonda E. Ries, Janine O. Ilagan, Derek L. Stirewalt, Soheil Meshinchi, Robert K. Bradley,

Single-cell and spatial transcriptomics

Significance An important goal of cancer biology is to identify molecular differences between normal and cancer cells. Accordingly, many large-scale initiatives to characterize both solid and liquid tumor samples with genomics technologies are currently underway. Here, we show that standard blood collection procedures cause rapid changes to the transcriptomes of hematopoietic cells. The resulting transcriptional and posttranscriptional artifacts are visible in most published leukemia genomics datasets and hinder the identification and interpretation of cancer-specific alterations.