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MTO: the second member of a Drosophila dual copper‐thionein system

2002; Wiley; Volume: 533; Issue: 1 Linguagem: Inglês

10.1016/s0014-5793(02)03754-7

1873-3468

Jordi Domènech-Casal, Òscar Palacios, Laura Villarreal, P. Gonzàlez-Duarte, Mercè Capdevila, Sı́lvia Atrian,

Drug Transport and Resistance Mechanisms

Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc‐ or copper‐thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper‐thionein, putting Drosophila forward as the only metazoan in which any zinc‐thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal‐MTO aggregates.