Portal de Periódicos da CAPES

A non-genomic signaling pathway shut down by mating changes the estradiol-induced gene expression profile in the rat oviduct

2009; Bioscientifica; Volume: 139; Issue: 3 Linguagem: Inglês

10.1530/rep-09-0218

1741-7899

Alexis Parada‐Bustamante, Pedro A. Orihuela, Mariana Ríos, Catherina A. Cuevas, María L. Oróstica, Luís Velásquez, Manuel Villalón, Horacio B. Croxatto,

Reproductive Biology and Fertility

Estradiol (E 2 ) accelerates oviductal egg transport through intraoviductal non-genomic pathways in unmated rats and through genomic pathways in mated rats. This shift in pathways has been designated as intracellular path shifting (IPS), and represents a novel and hitherto unrecognized effect of mating on the female reproductive tract. We had reported previously that IPS involves shutting down the E 2 non-genomic pathway up- and downstream of 2-methoxyestradiol. Here, we evaluated whether IPS involves changes in the genomic pathway too. Using microarray analysis, we found that a common group of genes changed its expression in response to E 2 in unmated and mated rats, indicating that an E 2 genomic signaling pathway is present before and after mating; however, a group of genes decreased its expression only in mated rats and another group of genes increased its expression only in unmated rats. We evaluated the possibility that this difference is a consequence of an E 2 non-genomic signaling pathway present in unmated rats, but not in mated rats. Mating shuts down this E 2 non-genomic signaling pathway up- and downstream of cAMP production. The Star level is increased by E 2 in unmated rats, but not in mated rats. This is blocked by the antagonist of estrogen receptor ICI 182 780, the adenylyl cyclase inhibitor SQ 22536, and the catechol- O -methyltransferase inhibitor, OR 486. These results indicate that the E 2 -induced gene expression profile in the rat oviduct differs before and after mating, and this difference is probably mediated by an E 2 non-genomic signaling pathway operating on gene expression only in unmated rats.