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Preconditioning and postconditioning: The essential role of the mitochondrial permeability transition pore

2007; Oxford University Press; Volume: 75; Issue: 3 Linguagem: Inglês

10.1016/j.cardiores.2007.04.022

1755-3245

Shiang Y. Lim, Sean M. Davidson, Derek J. Hausenloy, Derek M. Yellon,

Cardiac Arrest and Resuscitation

The opening of the mitochondrial permeability transition pore (mPTP) at the time of myocardial reperfusion is a critical determinant of cell death. Emerging studies suggest that suppression of mPTP opening may underlie the cardioprotection elicited by both ischemic preconditioning (IPC) and postconditioning (IPost). To further evaluate the role of the mPTP in cardioprotection, we hypothesized that hearts deficient in cyclophilin-D (CYP-D-/-), a key component of the mPTP, will be resistant to cardioprotection conferred by ischemic and pharmacological preconditioning and postconditioning.Male/female wild type or CYP-D-/- mice were subjected to 30 min of ischemia and 120 min of reperfusion. In wild type mice subjected to in vivo myocardial ischemia-reperfusion injury, a significant reduction in myocardial infarct size was observed with the following treatments (n>/=6/group; P /=6/group: P>0.05).This study demonstrates that the mPTP plays a critical role in the cardioprotection elicited by ischemic and pharmacological preconditioning and postconditioning.