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CE to monitor endotoxins by protein complexation

2006; Wiley; Volume: 27; Issue: 21 Linguagem: Inglês

10.1002/elps.200600276

1522-2683

Anikó Kilár, Béla Kocsis, Ildikó Kustos, Ferenc Kilár, Stellan Hjertén,

Analytical Chemistry and Chromatography

Abstract A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between “smooth” and “rough” lipopolysaccharides from Salmonella minnesota (wild‐type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.