Portal de Periódicos da CAPES

Mimetic ligand-based affinity purification of immune complexes and immunoconjugates

1998; Elsevier BV; Volume: 706; Issue: 2 Linguagem: Inglês

10.1016/s0378-4347(97)00441-6

1872-812X

Dan Xu, Béatrice Leveugle, Fernando Kreutz, M.R. Suresh,

Glycosylation and Glycoproteins Research

We developed a simple purification method to purify alkaline phosphatase/anti-alkaline phosphatase IgG as immune complexes using mimetic affinity chromatography wherein the antibody was either a monospecific antibody, a bispecific antibody or a commercial polyclonal IgG conjugated with alkaline phosphatase (AP-IgG) covalently. The immune complexes or conjugates were efficiently bound on the mimetic Blue A6XL column and eluted under mild conditions (5-20 mM phosphate buffer). A similar strategy of purifying peroxidase/anti-peroxidase antibody complexes was also successfully demonstrated using the mimetic Red 3 column. Mimetic affinity chromatography thus appears to be a simple method to purify the desired monospecific or bispecific antibodies from the respective hybridomas and quadromas.