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Prediction of Protein Secondary Structure by Combining Nearest-neighbor Algorithms and Multiple Sequence Alignments

1995; Elsevier BV; Volume: 247; Issue: 1 Linguagem: Inglês

10.1006/jmbi.1994.0116

1089-8638

Asaf Salamov, Victor Solovyev,

RNA and protein synthesis mechanisms

Recently Yi & Lander used a neural network and nearest and nearest-neighbor method with a scoring system that combined a sequence-similarity matrix with the local structural environment scoring scheme described by Bowie and co-workers for predicting protein secondary structure. We have improved their scoring system by taking into consideration N and C-terminal positions of α-helices and β-strands and also β-turns as distinctive types of secondary structure. Another improvement, which also decreases the time of computation, is performed by restricting a data base with a smaller subset of proteins that are similar with a query sequence. Using multiple sequence alignments rather than single sequences and a simple jury decision procedure our method reaches a sustained overall three-state accuracy of 72.2%, which is better than that observed for the most accurate multi-layered neural-network approach, tested on the same data set of 126 non-homologous protein chains.