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Isolation of DNA Sequences Amplified at Chromosome 19q13.1–q13.2 Including theAKT2Locus in Human Pancreatic Cancer

1996; Elsevier BV; Volume: 225; Issue: 3 Linguagem: Inglês

10.1006/bbrc.1996.1280

1090-2104

Wataru Miwa, Jun Yasuda, Yoshinori Murakami, Kazuo Yashima, Kokichi Sugano, Teruaki Sekine, Akira Kono, Shinichi Egawa, Ken Yamaguchi, Yoshihide Hayashizaki, Takao Sekiya,

RNA Interference and Gene Delivery

In the human pancreatic cancer cell line PANC1, we detected several DNA fragments with abnormally intensified signals by restriction landmark genomic scanning. Major five of these fragments were cloned. All of the cloned fragments were mapped at the 19q13.1-13.2 region where the AKT2 oncogene was located. Southern blotting using the cloned DNA fragments and a fragment of AKT2 cDNA as probes revealed that the AKT2 gene was amplified in 3 of 12 pancreatic cancer cell lines analyzed including PANC1 and in 3 of 20 primary pancreatic cancers. The AKT2 gene was overexpressed in the 3 cell lines with the amplified gene. The results suggest that the AKT2 gene is a candidate oncogene activated by amplification in some human pancreatic cancers.