A new synthesis of [3-11C]pyruvic acid using alanine racemase
1998; Elsevier BV; Volume: 49; Issue: 12 Linguagem: Inglês
10.1016/s0969-8043(98)00052-9
ISSN1872-9800
AutoresM. Ikemoto, Makoto Sasaki, Terushi Haradahira, Eri Okamoto, Hironori Omura, Y. Furuya, Yasuyoshi Watanabe, Katsuhiko Suzuki,
Tópico(s)Enzyme Structure and Function
ResumoAbstract The synthesis of [3-11C]pyruvic acid was attempted by two reaction systems (A: alanine racemase and d -amino acid oxidase, B: alanine racemase and l -alanine dehydrogenase) utilizing a new thermostable enzyme, alanine racemase. Conversion rates from d , l -[3-11C]alanine to [3-11C]pyruvic acid were almost 100% in both methods. Similar results were obtained with immobilized enzymes packed in a single column. Furthermore, the same column could be used repeatedly without a remarkable decrease of the [3-11C]pyruvic acid yield. Various matrices were tested for the immobilizing enzyme, and Aminopropyl-CPG was concluded to be the most suitable since the loss of the enzyme activity was the least in the studied matrices.
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