Produção Nacional
Revisado por pares
Statistical experimental design to assess the influence of enzymes of nematophagous fungi versus helminths
2014; Elsevier BV; Volume: 97; Issue: 3 Linguagem: Inglês
10.1016/j.rvsc.2014.09.005
ISSN1532-2661
AutoresFábio Ribeiro Braga, Filippe Elias de Freitas Soares, Juliana Milani Araújo, Leandro Abreu da Fonseca, Emy Hiura, Maylla Garschagen Gava, Fernanda Toledo Vieira, Jeanne Saraiva da Paz, Lorendane Milena de Carvalho, João Victor Faccini, José Humberto de Queiróz, Jackson Víctor de Araújo,
Tópico(s)Insect Pest Control Strategies
ResumoThe present work used Plackett–Burman experimental design to assess the influence of enzymes of nematophagous fungi versus Strongyloides westeri and trichostrongylides larvae and Platynosomum fastosum eggs. The variables studied in the Plackett–Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents of S. westeri and trichostrongylides larvae, and P. fastosum eggs. All tested enzymes had a significant effect (P < 0.05) on the destruction of S. westeri larvae. Furthermore, only VC4 and AC001 proteases showed a significant effect (P < 0.05) on the destruction of trichostrongylides larvae. On the other hand, chitinases of VC4 showed the highest significance (P < 0.05) on the destruction of P. fastosum eggs. It is proposed that statistical planning for the use of enzymes derived from nematophagous fungi is a viable way to elucidate some questions about their mechanism of action.
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