Global analysis of fluorescence decay surfaces: excited-state reactions
1985; Elsevier BV; Volume: 120; Issue: 4-5 Linguagem: Inglês
10.1016/0009-2614(85)85642-6
ISSN1873-4448
AutoresJoseph Beechem, Marcel Ameloot, Ludwig Brand,
Tópico(s)Spectroscopy and Laser Applications
ResumoA procedure is proposed for the analysis of fluorescence decay data for systems undergoing excited-state reactions. The rate constants describing the excited-state reactions as well as the spectra associated with each emitting state are recovered in a single analysis step. The method incorporates the simultaneous nonlinear analysis of a multidimensional data surface of time, multiple wavelenghts and concentrations. Complex excited-state reaction schemes can be tested and highly overlapping spectra can be resolved.
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