In vitro bioconversion of [14C]androstenedione by testes of the Siamese fighting fish Betta splendens regan (Anabantoidei, Belontiidae)
1985; Elsevier BV; Volume: 58; Issue: 3 Linguagem: Inglês
10.1016/0016-6480(85)90121-2
ISSN1095-6840
AutoresThomas Leitz, Rudolf Reinboth,
Tópico(s)Reproductive biology and impacts on aquatic species
ResumoMinced testes of the Siamese fighting fish Betta splendens were incubated with [14C]androstenedione at 27°C for 15, 30, 60, and 120 min. The metabolic products were characterized by paper and thin-layer chromatography, derivative formation, and eventually by crystallization to constant specific activity. After 2 hr of incubation 80.5% of total radioactivity was converted to 11-oxygenated androgens. 11-Ketotestosterone was the main metabolite (56.2%). Our data suggest the existence of two biosynthetic pathways for the formation of 11-ketotestosterone from androstenedione. The sequence androstenedione → testosterone → 11β-hydroxytestosterone → 11-ketotestosterone predominates. To a lesser extent 11β-hydroxylation takes place as the first step—followed by formation of 11β-hydroxytestosterone and its subsequent oxidation to 11-ketotestosterone.
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