Determination of chlorophyll in marine waters: intercomparison of a rapid HPLC method with full HPLC, spectrophotometric and fluorometric methods
1986; Elsevier BV; Volume: 19; Issue: 3 Linguagem: Inglês
10.1016/0304-4203(86)90024-1
ISSN1872-7581
AutoresAndrew Murray, CF Gibbs, AR Longmore, Dianne J. Flett,
Tópico(s)Advanced Chemical Sensor Technologies
ResumoA statistical comparison was made between chlorophyll measurements made on a suite of marine samples by four methods. The methods were one complete and one rapid high-performance liquid chromatographic (HPLC) method, a fluorometric method and the traditional spectrophotometric method. Generally good agreement was found, provided that consideration was given to the presence of chlorophyllide a and allomers of chlorophyll a as determined by the HPLC methods. The rapid HPLC method (10 min per sample) is recommended as a convenient and accurate alternative to traditional methods for chlorophylls.
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