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Detection of aptamer–protein interactions using QCM and electrochemical indicator methods

2004; Elsevier BV; Volume: 15; Issue: 2 Linguagem: Inglês

10.1016/j.bmcl.2004.10.083

1464-3405

Tibor Hianik, Veronika Ostatná, Zuzana Zajacová, E. E. Stoikova, Gennady Evtugyn,

RNA and protein synthesis mechanisms

We report novel method of detection thrombin–aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to a thrombin. The binding of thrombin to an aptamers has been detected also by QCM method in flow measuring cell. We showed that using MB it is possible to detect thrombin with high sensitivity and selectivity.