Artigo Acesso aberto

Toxic action of copper on the membrane system of a marine diatom measured by flow cytometry

1996; Wiley; Volume: 25; Issue: 1 Linguagem: Inglês

10.1002/(sici)1097-0320(19960901)25

ISSN

1097-0320

Autores

Ángeles Cid, Pablo Fidalgo, Concepción Herrero, Julio Abalde,

Tópico(s)

Marine Biology and Environmental Chemistry

Resumo

CytometryVolume 25, Issue 1 p. 32-36 Original ArticleFree Access Toxic action of copper on the membrane system of a marine diatom measured by flow cytometry Angeles Cid, Corresponding Author Angeles Cid Laboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainLaboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainSearch for more papers by this authorPablo Fidalgo, Pablo Fidalgo Laboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainSearch for more papers by this authorConcepción Herrero, Concepción Herrero Laboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainSearch for more papers by this authorJulio Abalde, Julio Abalde Laboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainSearch for more papers by this author Angeles Cid, Corresponding Author Angeles Cid Laboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainLaboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainSearch for more papers by this authorPablo Fidalgo, Pablo Fidalgo Laboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainSearch for more papers by this authorConcepción Herrero, Concepción Herrero Laboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainSearch for more papers by this authorJulio Abalde, Julio Abalde Laboratorio de Microbiologia, Departamento de Biologia Celular y Molecular, Universidade da Coruña, Coruña, SpainSearch for more papers by this author First published: 1 September 1996 https://doi.org/10.1002/(SICI)1097-0320(19960901)25:1 3.0.CO;2-GCitations: 65AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Flow cytometric measurements were used to investigate the toxic action of copper on some Phaeodactylum tricornutum membrane systems. Throughout the time of metal exposure, the percentage of viable cells decreased as copper concentration increased. The forward scatter signal increased as a result of copper exposure. After 72 h of metal exposure, cultures with 0.5 and 1 mg l−1 of copper showed an important increase in the peroxidase activity in comparison with control cells. Cells cultured with copper presented alterations in the membrane potential, increasing as copper concentration increased, after 96 h of metal exposure. Results obtained in this work showed that copper induced a degenerative process in P. tricornutum cells, closely related with alterations or disorders in membrane systems. © 1996 Wiley-Liss, Inc. 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Citing Literature Volume25, Issue11 September 1996Pages 32-36 ReferencesRelatedInformation

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