The Cauliflower Mosaic Virus 35 S Promoter: Combinatorial Regulation of Transcription in Plants
1990; American Association for the Advancement of Science; Volume: 250; Issue: 4983 Linguagem: Inglês
10.1126/science.250.4983.959
ISSN1095-9203
AutoresPhilip N. Benfey, Nam‐Hai Chua,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoAppropriate regulation of transcription in higher plants requires specific cis elements in the regulatory regions of genes and their corresponding trans-acting proteins. Analysis of the cauliflower mosaic virus (CaMV) 35S promoter has contributed to the understanding of transcriptional regulatory mechanisms. The intact 35S promoter confers constitutive expression upon heterologous genes in most plants. Dissection into subdomains that are able to confer tissue-specific gene expression has demonstrated that the promoter has a modular organization. When selected subdomains are combined, they confer expression not detected from the isolated subdomains, suggesting that synergistic interactions occur among cis elements. The expression patterns conferred by specific combinations of 35S subdomains differ in tobacco and petunia. This indicates that a combinatorial code of cisregulatory elements may be interpreted differently in different species.
Referência(s)