Portal de Periódicos da CAPES

Identification of furanose and pyranose ring forms of carbohydrates by methylation, gas-liquid chromatography and mass spectrometry

1987; Elsevier BV; Volume: 396; Linguagem: Inglês

10.1016/s0021-9673(01)94050-6

1873-3778

John H. Pazur, Frank J. Miskiel, Belin Liu,

Microbial Metabolites in Food Biotechnology

New data on the furanose and pyranose ring transformations of glucose, arabinose and galactose in dimethyl sulfoxide solvent have been obtained by the combined technique of methylation, gas-liquid chromatography and mass spectrometry. The ratios of the furanose to pyranose ring forms in the monosaccharides were 1:1.7 for arabinose, 1:1.2 for galactose and 1:99 for glucose. The method has been used to detect furanose and pyranose ring forms in the reducing units of two disaccharides of galactose and arabinose. Thus, the furanose and pyranose ratios of the reducing units of α- and β-d-galactopyranosyl-(1,3)-d- or -l-arabinose are 2:1 for the α-linked and 1:4 for the β-linked disaccharide. The acyclic form of arabinose could also be detected by the analytical technique. The combined methylation, gas-liquid chromatography and mass spectrometry procedure should be useful for determining the ring forms of the reducing units of other oligosaccharides and for identifying acyclic forms of reducing sugars.