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Chondroitin sulfate proteoglycan expression pattern in hippocampal development: Potential regulation of axon tract formation

2000; Wiley; Volume: 424; Issue: 3 Linguagem: Inglês

10.1002/1096-9861(20000828)424

1096-9861

Mark T. Wilson, Diane M. Snow,

Nerve injury and regeneration

Journal of Comparative NeurologyVolume 424, Issue 3 p. 532-546 Research Article Chondroitin sulfate proteoglycan expression pattern in hippocampal development: Potential regulation of axon tract formation Mark T. Wilson, Mark T. Wilson Department of Anatomy and Neurobiology, University of Kentucky, Lexington, Kentucky 40536-0298Search for more papers by this authorDiane M. Snow, Corresponding Author Diane M. Snow [email protected] Department of Anatomy and Neurobiology, University of Kentucky, Lexington, Kentucky 40536-0298Department of Anatomy and Neurobiology, Chandler Medical Center, University of Kentucky, Lexington, KY 40536-0298Search for more papers by this author Mark T. Wilson, Mark T. Wilson Department of Anatomy and Neurobiology, University of Kentucky, Lexington, Kentucky 40536-0298Search for more papers by this authorDiane M. Snow, Corresponding Author Diane M. Snow [email protected] Department of Anatomy and Neurobiology, University of Kentucky, Lexington, Kentucky 40536-0298Department of Anatomy and Neurobiology, Chandler Medical Center, University of Kentucky, Lexington, KY 40536-0298Search for more papers by this author First published: 18 July 2000 https://doi.org/10.1002/1096-9861(20000828)424:3 3.0.CO;2-ZCitations: 62Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract A variety of molecular influences in the extracellular matrix (ECM) interact with developing axons to guide the formation of hippocampal axon pathways. One of these influences may be chondroitin sulfate proteoglycans (CSPGs), which are known to inhibit axonal extension during development and following central nervous system injury. In this study, we examined the role of CSPGs and cell adhesion molecules in the regulation of axon tract formation during hippocampal development. We used indirect immunofluorescence to examine the developmental pattern of CSPG expression relative to axon tracts that express the cell adhesion molecule L1. Additionally, we used dissociated and explant cell cultures to examine the effects of CSPGs on hippocampal axon development in vitro. In vivo, we found that the CSPG neurocan is expressed throughout the alveus, neuropil layers, and parts of the dentate gyrus from E16 to P2. The CSPG phosphacan is expressed primarily in the neuropil layers at postnatal stages. After E18, intense labeling of neurocan was observed in regions of the alveus surrounding L1-expressing axon fascicles. In vitro, axons from brain regions that project through the alveus during development would not grow across CSPG substrata, in a concentration-dependent manner. In addition, hippocampal axons from dissociated neuron cultures only traveled across CSPG substrata as fasciculated axon bundles. These findings implicate CSPG in the regulation of axon trajectory and fasciculation during hippocampal axon tract formation. J. Comp. Neurol. 424:532–546, 2000. © 2000 Wiley-Liss, Inc. LITERATURE CITED Anderson RB, Walz A, Holt CE, Key B. 1998. Chondroitin sulfates modulate axon guidance in embryonic Xenopus brain. Dev Biol 202: 235–243. 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