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Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA

2008; Elsevier BV; Volume: 155; Issue: 2 Linguagem: Inglês

10.1016/j.jviromet.2008.10.016

1879-0984

Zainah Saat, Ahmad Wahab, M Mariam, M.K. Fauziah, A H Khairul, I. Roslina, A. Sairulakhma, S.S. Kadimon, Mariel Jais, Kaw Bing Chua,

Malaria Research and Control

The performance of a commercial immunochromatography test for rapid detection of dengue NS1 antigen present in serum or plasma of patients was evaluated against a commercial dengue NS1 antigen-capture ELISA. The rapid immunochromatography test gave an overall sensitivity of 90.4% with a specificity of 99.5%. The sensitivity was highest for serum samples from which virus was isolated (96.3%) and lowest for those from which virus was not isolated and RT-PCR was negative (76.4%). The sensitivity was significantly higher for serum samples from patients with acute primary dengue (92.3%) than those from patients with acute secondary dengue (79.1%). The positive predictive value and negative predictive value of this commercial immunochromatography test were 99.6% and 87.9% respectively.