Portal de Periódicos da CAPES

Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome

2003; Centers for Disease Control and Prevention; Volume: 9; Issue: 9 Linguagem: Inglês

10.3201/eid0909.030304

1080-6059

Paul K.S. Chan, John S. Tam, Ching‐Wan Lam, Edward Wai‐Chi Chan, Alan Wu, Chi Kong Li, Thomas A. Buckley, King-Cheung Ng, Gavin M. Joynt, Frankie Wai Tsoi Cheng, Ka‐Fai To, Nelson Lee, David S.C. Hui, Jo L.K. Cheung, Ida Miu‐Ting Chu, Esther Liu, Enders K. Ng, Joseph J.�Y. Sung,

Viral Infections and Immunology Research

We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.