Portal de Periódicos da CAPES

Homogeneous time resolved fluorescence resonance energy transfer using rare earth cryptates as a tool for probing molecular interactions in biology

2001; Elsevier BV; Volume: 57; Issue: 11 Linguagem: Inglês

10.1016/s1386-1425(01)00493-0

1873-3557

Hervé Bazin, Marc Préaudat, Eric Trinquet, Gérard Mathis,

Lanthanide and Transition Metal Complexes

A homogeneous assay technology using time resolved fluorescence and fluorescence resonance energy transfer is described. A new class of fluorescent complexes, the cryptates, have been used as fluorescent donor with cross-linked allophycocyanin as acceptor. This new donor/acceptor shows an exceptionally high Förster distance R0 of 9 nm. This allows to build up a set of strategies to probe the interactions of biomolecules in biology, particularly for high throughput screening applications. In this article, we describe the basics of the technology and review applications developed for studying different key molecular interactions involved in cellular processes.