Produção Nacional
Revisado por pares
Biodistribution study and identification of inflammation sites using 99mTc-labelled stealth pH-sensitive liposomes
2008; Lippincott Williams & Wilkins; Volume: 29; Issue: 1 Linguagem: Inglês
10.1097/mnm.0b013e3282f1bc0d
ISSN1473-5628
AutoresVildete Aparecida Sousa Carmo, Cristiano S. Ferrari, Eduardo C. O. Reis, Gilson Andrade Ramaldes, Maira Alves Pereira, Mônica Cristina Oliveira, Valbert Nascimento Cardoso,
Tópico(s)Bone and Joint Diseases
ResumoTo investigate the biodistribution and the ability of stealth pH-sensitive liposomes radiolabelled with 99mTc to identify inflammatory regions in a rat focal inflammation model.Preformed glutathione-containing stealth pH-sensitive liposomes were labelled with 99mTc-hexamethylpropylene amine oxime (99mTc-HMPAO). The 99mTc-HMPAO radiolabelled stealth pH-sensitive liposomes (99mTc-SpHL) were administered intravenously in Wistar male rats with inflammation induced by injection subplantar of carrageenan in the right foot. At pre-established time intervals the animals were anaesthetized and tissues were removed and analysed for 99mTc content using an automatic scintillation apparatus. Scintigraphic imaging was also performed after 2, 4 and 8 h of intravenous injection of 99mTc-SpHL.The 99mTc-SpHL was significantly taken up by the spleen (19.21+/-2.98%ID/g at 30 min post-injection). Low radioactivity levels were found in the liver, lungs, and kidney. Moreover, the 99mTc-SpHL uptake was significantly higher in the inflamed foot when compared to the respective control (0.386+/-0.059 and 0.215+/-0.018%ID/g at 2 h post-injection, respectively). As early as 30 min after administration of 99mTc-SpHL, the focus of inflammation could be visualized scintigraphically. The value of the inflammatory and non-inflammatory site radioactivity counting ratio was greater than 5.This result indicates that the 99mTc-SpHL presents a high tropism for inflammatory regions and may be useful as a radiopharmaceutical to identify these foci.
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