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Pyridinium Trifluoroacetate/ N -Methylimidazole as an Efficient Activator for Oligonucleotide Synthesis via the Phosphoramidite Method

2000; American Chemical Society; Volume: 4; Issue: 3 Linguagem: Inglês

10.1021/op9900378

1520-586X

Alessandra Eleuteri, Daniel C. Capaldi, Achim H. Krotz, Douglas L. Cole, Vasulinga T. Ravikumar,

Biopolymer Synthesis and Applications

A new activator is reported for coupling phosphoramidites to a free 5'-hydroxyl group during oligonucleotide synthesis. Pyridinium trifluoroacetate/N-methyl imidazole is a remarkably efficient replacement for 1H-tetrazole in the solid-supported synthesis of oligonucleotides. This reagent is safe and inexpensive, is not moisture-sensitive, and is soluble in acetonitrile.