Produção Nacional
Revisado por pares
Experimental mixture design as a tool to enhance glycosyl hydrolases production by a new Trichoderma harzianum P49P11 strain cultivated under controlled bioreactor submerged fermentation
2012; Elsevier BV; Volume: 132; Linguagem: Inglês
10.1016/j.biortech.2012.11.087
ISSN1873-2976
AutoresPriscila da Silva Delabona, Cristiane S. Farinas, Deise Juliana da Silva Lima, José Geraldo da Cruz Pradella,
Tópico(s)Enzyme Production and Characterization
ResumoThis work investigates the glycosyl hydrolase (GH) profile of a new Trichoderma harzianum strain cultivated under controlled bioreactor submerged fermentation. The influence of different medium components (delignified steam-exploded sugarcane bagasse, sucrose, and soybean flour) on GH biosynthesis was assessed using experimental mixture design (EMD). Additionally, the effect of increased component concentrations in culture media selected from the EMD was studied. It was found that that a mixed culture medium could significantly maximize GH biosynthesis rate, especially for xylanase enzymes which achieved a 2-fold increment. Overall, it was demonstrated that T. harzianumP49P11 enzymes have a great potential to be used in the deconstruction of biomass.
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