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Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats

2006; Elsevier BV; Volume: 41; Issue: 6 Linguagem: Inglês

10.1016/j.exger.2006.03.006

1873-6815

Gordana Leposavić, Vesna Pešić, Duško Kosec, Katarina Radojević, Nevena Arsenović-Ranin, Ivan Pilipović, Milica Perišić Nanut, Bosiljka Plećaš‐Solarović,

Immunotherapy and Immune Responses

To elucidate the effects of ageing on T-cell-maturation, in 3- and 18-month-old rats, we analysed the expression of: (i) CD4/CD8/TCRαβ and (ii) Thy-1, which is supposed to be a regulator of TCRαβ signalling, and thereby the thymocyte selection thresholds. Since an essential role for TCRαβ signalling in the development of CD4+25+Treg-cells was suggested, the frequency of these cells was also quantified. We demonstrated that, as for mice, early thymocyte differentiational steps within the CD4-8- double negative (DN) developmental stage are age-sensitive. Furthermore, we revealed that TCRαβ-dependent stages of T-cell development are affected by ageing, most likely due to an impaired expression of Thy-1 on TCRαβlow thymocytes entering selection processes. The diminished frequency of the post-selection CD4+8+ double positive (DP) cells in aged rats, together with an overrepresentation of mature single positive (SP) cells, most probably suggests more efficient differentiational transition from the DP TCRαβhigh to the SP TCRαβhigh developmental stage, which is followed by an increase in pre-migration proliferation of the mature SP cells. Moreover, the study indicated impaired intrathymic generation of CD4+25+Treg-cells in aged rats, thus providing a possible explanation for the increased frequency of autoimmune diseases in ageing.