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Fragmentation studies of peptides: The formation of Y ions

1992; Wiley; Volume: 6; Issue: 2 Linguagem: Inglês

10.1002/rcm.1290060205

1097-0231

Peter T.M. Kenny, Kyosuke Nomoto, Ron Orlando,

Advanced Proteomics Techniques and Applications

Abstract The mechanism for the formation of y ions in the collision‐induced dissociation (CID) spectra of protonated peptides produced by fast‐atom bombardment was investigated by tandem mass spectrometry and deuterium labelling studies. The results show that a hydrogen atom attached to nitrogen and not to carbon migrates during cleavage of the amide bond. A mechanism based on these results is presented.