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Isolation and Identification of Sphingomonas sp. That Yields tert -Octylphenol Monoethoxylate under Aerobic Conditions

2005; Oxford University Press; Volume: 69; Issue: 7 Linguagem: Inglês

10.1271/bbb.69.1226

1347-6947

Eriko Nishio, Hiromichi Yoshikawa, Manabu Wakayama, Hiroto Tamura, Shirô Morita, Yoshifumi Tomita,

Marine Biology and Environmental Chemistry

Topsoil samples were collected from eight golf courses in Yamaguchi Prefecture, Japan, and enrichment cultures were carried out with a basal-salt medium containing 0.2% 4-tert-octylphenol polyethoxylate (OPPEO) as sole carbon source. OPPEO-degrading activity was detected in one of the samples, from which a strain of OPPEO-degrading bacterium was isolated. The isolated bacterium grew on a nutritionally enriched medium (NE medium) containing 0.2% OPPEO as sole carbon source, and accumulated 4-tert-octylphenol diethoxylate (OP2EO) (63%), 4-tert-octylphenol triethoxylate (OP3EO) (14%), and 4-tert-octylphenol monoethoxylate (OP1EO) (2%) after 7 d cultivation under aerobic conditions. The addition of clay mineral (vermiculite) to the medium accelerated the degradation of OP2EO (40%) and OP3EO (4%) to OP1EO (23%). This is the first report about bacteria that can degrade OPPEO to OP1EO under aerobic conditions. The strain was identified as Sphingomonas macrogoltabidus, based on the homology of a 16S rDNA sequence.