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Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup

1990; Lippincott Williams & Wilkins; Volume: 4; Issue: 12 Linguagem: Inglês

10.1097/00002030-199012000-00012

1473-5571

Tomoyuki Miura, Jun-ichi Sakuragi, Meiko Kawamura, Masashi Fukasawa, Etsuko N. Moriyama, Takashi Gojobori, Koh-ichi Ishikawa, J. A. A. Mingle, Victor B.A. Nettey, Hirofumi Akari, Masayoshi Enami, Hajime Tsujimoto, Masanori Hayami,

Herpesvirus Infections and Treatments

We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIVmac/SIVsm in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.