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A highly sensitive and specific assay for vertebrate collagenase

1981; Wiley; Volume: 16; Issue: 4 Linguagem: Inglês

10.1111/j.1600-0765.1981.tb00993.x

1600-0765

Jaro Sodek, S. Hurum, Jun Feng,

dental development and anomalies

A highly sensitive and specific assay for vertebrate collagenase has been developed using a [ 14 C]‐labeled collagen substrate and a combination of SDS‐PAGE (sodium dodecyl sulfate‐polyacrylamide gel electrophoresis) and fluorography to identify and quantitate the digestion products. The assay was sufficiently sensitive to permit the detection and quantitation of collagenase activity in 0.1 μl of gingival sulcal fluid, and in samples of cell culture medium without prior concentration. The assay has also been used to detect the presence of inhibitors of collagenolytic enzymes in various cell culture fluids.