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Spectrophotometric flow injection determination of ?ascorbic acid with a packed reactor containing ferric hydroxide

1998; Elsevier BV; Volume: 47; Issue: 1 Linguagem: Inglês

10.1016/s0039-9140(98)00047-2

1873-3573

Arnaldo César Pereira,

Analytical Chemistry and Chromatography

A flow injection system with spectrophotometric detection is proposed for determining l-ascorbic acid in pharmaceutical formulations. In this system a column containing Fe(OH)(3) immobilized in polyester resin (packed reactor) is inserted before the detector. Fe(III)-1,10-phenanthroline complex is reduced by l-ascorbic acid to produce Fe(II)-1,10-phenanthroline complex which is monitored at 510 nm. Under the optimum analytical conditions, the linearity of the calibration equation for l-ascorbic acid ranged from 5.0x10(-6) to 6.0x10(-5) M of added amount. The detection limit was 5.0x10(-7) M and recoveries between 98.5-102.0% were obtained. No interference was observed from the common excipients of pharmaceutical formulations and other active substances such as acetylsalicylic acid, caffeine and thiamine.