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Lipoproteins I and II from the hemolymph of the blue crab Callinectes sapidus : Lipoportein II associated with vitellogenesis

1988; Wiley; Volume: 248; Issue: 3 Linguagem: Inglês

10.1002/jez.1402480306

1097-010X

Richard F. Lee, Donald L. Puppione,

Aquaculture Nutrition and Growth

Abstract Two different high density lipoproteins were isolated and characterized in the hemolymph of blue crabs ( Callinectes sapidus ). Lipoprotein I was found in both sexes and comprised one peptide (112,000 daltons) and lipid, primarily phosphtidyl choline. Lipoprotein I properties included a hydrated density of 1.12 g/ml, sedimentation coefficient of 5.4 Svedberg flotation units and a protein to lipid ratio of 1.0. This lipoprotein is assumed to have a role in transporting lipid from the hepatopancreas to other tissues. Lipoprotein II had three peptides (190,000, 107,000 and 78,000 daltons), phosphatidyl choline as the major lipid, a hydrated density of 1.16 g/ml, a sedimentation coefficient of 10.4 Svedberg flotation units, a protein to lipid ratio of 1.1, and a yellow to orange color due to a mixture of carotenoids. Lipoprotein II was found only in female crabs undergoing vitellogenesis. Lipoprotein II appeared after 30 days in females that had mated and completed their final molt. The maximum concentration was reached in 50 to 60 days. Lipoprotein II was not present in recently molted females in a collection of crabs from one esturine river but was present in all other stages with highest concentrations (up to 8 mg/ml hemolymph) in stages 5 and 6 (females with large, well developed ovaries). Crab ovaries had a lipoprotein similar to hemolymph Lipoprotein II. Lipoprotein II probably plays an important role in carrying lipid and specific peptides to ovarian tissues. Crustacean hemolymph lipoproteins show some similarities to insect lipophorins, but are quite different from vertebrate lipoproteins.