Nalidixic Acid Resistance Increases Sensitivity of Escherichia coli O157:H7 to Ionizing Radiation in Solution and on Green Leaf Lettuce
2005; Wiley; Volume: 70; Issue: 2 Linguagem: Inglês
10.1111/j.1365-2621.2005.tb07101.x
ISSN1750-3841
Autores Tópico(s)Insect and Pesticide Research
ResumoJournal of Food ScienceVolume 70, Issue 2 p. M121-M124 Nalidixic Acid Resistance Increases Sensitivity of Escherichia coli O157:H7 to Ionizing Radiation in Solution and on Green Leaf Lettuce Brendan A. Niemira, Brendan A. Niemira The author is with Food Safety Intervention Technologies Research Unit, U.S. Dept. of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038. Direct inquiries to author Miemira (E-mail: [email protected]).Search for more papers by this author Brendan A. Niemira, Brendan A. Niemira The author is with Food Safety Intervention Technologies Research Unit, U.S. Dept. of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038. Direct inquiries to author Miemira (E-mail: [email protected]).Search for more papers by this author First published: 31 May 2006 https://doi.org/10.1111/j.1365-2621.2005.tb07101.xCitations: 14AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract ABSTRACT: Nalidixic acid resistance has been used as a selective marker for studies of pathogen-inoculated fruits and vegetables. Three nalidixic acid-sensitive outbreak strains of Escherichia coli O157:H7 were used to generate mutants resistant to nalidixic acid (NalR, 50 μg/mL) by successive culturing and selection in nalidixic acid-amended broth. The resistance to ionizing radiation of the parent and NalR strains was determined (a) in a phosphate buffer solution and (b) on green leaf lettuce. The NalR strains of each of the 3 isolates were significantly (P < 0.05) more sensitive to ionizing radiation than the nalidixic acid-sensitive (NalS) parent strains in both systems. D10 values (the amount of ionizing radiation required to achieve 1 log10 reduction) determined in buffer for the parent strains ranged from 0.18 to 0.33 kGy, whereas for the NalR strains, D10 were approximately 0.10 kGy, a reduction of up to 69%. When evaluated on green leaf lettuce, the D10 for the NalS strains was approximately 0.18 kGy as opposed to 0.10 to 0.12 kGy for the NalR strains, a reduction of up to 45%. The D10 values obtained on lettuce were significantly different than those obtained in buffer for 4 of the 6 isolates examined. The magnitude of the increase in radiation sensitivity resulting from resistance to nalidixic acid varied among the strains tested and also varied depending on the suspending medium. These results suggest that the use of nalidixic acid resistance as a selective marker may result in significant overestimates of the antimicrobial efficacy of ionizing radiation against E. coli O157:H7. References Blackburn CW , Davies AR. 1994 . Development of antibiotic-resistant strains for the enumeration of foodborne pathogenic bacteria in stored foods . Int J Food Microbiol 24 : 125 – 36 . Foley DM , Dufour A , Rodriguez L , Caporaso F , Prakash A. 2002 . Reduction of Escherichia coli O157:H7 in shredded iceberg lettuce by chlorination and gamma irradiation . Rad Phys Chem 63 : 391 – 6 . Georgopapadakou H , Dis BA , Angehrn P , Wick A , Olsen GL 1987 . 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