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Isotope effects of tritium in a ligand-antibody binding

1983; Pergamon Press; Volume: 34; Issue: 7 Linguagem: Inglês

10.1016/0020-708x(83)90072-8

1878-1284

Shu Xing, S.Z. Cekan,

Advanced biosensing and bioanalysis techniques

The affinity of testosterone labelled with 1,2,4 or 6 tritium atoms to testosterone antisera was examined. Scatchard plots indicated an increase of equilibrium constants with the number of tritium atoms per testosterone molecule. This behaviour was observed with all 3 antisera tested. It is speculated that the increase of affinity is due to a decrease of the amplitude of vibrations of tritium atoms, resulting in a decrease of vibrational repulsions.