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Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)

2012; Royal Society of Chemistry; Volume: 48; Issue: 21 Linguagem: Inglês

10.1039/c2cc17577k

1364-548X

Musleh M. Muthana, Jingyao Qu, Yanhong Li, Lei Zhang, Hai Yu, Ding Li, Hamed Malekan, Xi Chen,

Enzyme Production and Characterization

A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further chemical diversification of a UDP-mannose derivative resulted in the formation of UDP-N-acetylmannosamine.