A Model On-line Flow Injection Fluorescence Immunoassay Using a Protein a Immunoreactor and Lucifer Yellow
1993; Taylor & Francis; Volume: 26; Issue: 12 Linguagem: Inglês
10.1080/00032719308017972
ISSN1532-236X
AutoresDerek A. Palmer, Xuezhen Ren, Pilar Fernández Hernando, James N. Miller,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoAbstract A heterogeneous on-line fluorescence immunoassay has been developed for a model analyte (transferrin) using a flow injection analysis system containing a controlled pore glass protein A immunoreactor. Lucifer yellow VS (LYVS) a 4-aminonaphthalimide with a large stokes shift and pH independence was the fluorophore. For each assay the antibody-protein A reaction takes place at near neutral pH, and the complexes are eluted at acid pH. Transferrin levels in human serum has been determined by this method, and good within assay variations have been achieved.
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