Spectral phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR
2013; IOP Publishing; Volume: 1; Issue: 3 Linguagem: Inglês
10.1088/2050-6120/1/3/035001
ISSN2050-6120
AutoresFrancesco Cutrale, Anya Salih, Enrico Gratton,
Tópico(s)Photosynthetic Processes and Mechanisms
ResumoThe phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides easy identification of the spectral signatures of each photo-activatable protein.
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