Quantitative analysis of cytotoxicity and mutagenicity of benzo[a]pyrene in mammalian cells (CHO/HGPRT system)
1981; Elsevier BV; Volume: 34; Issue: 1 Linguagem: Inglês
10.1016/0009-2797(81)90084-3
ISSN1872-7786
AutoresRichard Machanoff, John P. O’Neill, Abraham W. Hsie,
Tópico(s)bioluminescence and chemiluminescence research
ResumoChinese hamster ovary (CHO) cells in cell culture were utilized to study the cytotoxic and mutagenic activity of benzo[a]pyrene (BP). A liver homogenate (S9) prepared from Arochlor 1254 induced male Sprague-Dawley rats was employed to mediate the biotransformation of the promutagen. The dependence of the induction of 6-thioguanine(TG)-resistant mutants on parameters such as BP concentration, amount of S9 protein, ionic composition of the S9 mix and treatment time was determined. Under optimal conditions with a 5-h treatment time, BP mutagenicity can be described as 185 mutants/106 cells per μM BP per mg/ml S9 protein.
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