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Hemocytes of Penaeid and Palaemonid shrimps: Morphology, cytochemistry, and hemograms

1989; Elsevier BV; Volume: 53; Issue: 1 Linguagem: Inglês

10.1016/0022-2011(89)90075-x

1096-0805

Angélique Tsing, Jean-Michel Arcier, Michel Brehélin,

Marine Bivalve and Aquaculture Studies

Hemocytes of Penaeus japonicus, Penaeus monodon, Macrobrachium rosenbergii, and Palaemon adspersus were separated into three cell types because of their ultrastructural features. These four crustacean species possessed hemocytes with small granules and hemocytes with large granules. In addition, hemocytes with a low level of differentiation were observed in the blood of P. japonicus and P. monodon. In P. monodon and M. rosenbergii the hemocytes with large granules were of two different subtypes due to the aspect of their inclusions. Some of the hemocytes with small granules, in P. adspersus, possessed vesicles which fused in large vacuoles and were not observed in the other species. In P. japonicus an acid phosphatase activity has been evidenced in granular hemocytes, especially in those with small granules. A phenoloxidase activity was confined to the cytosol of hemocytes with large granules. The three blood cell types of P. japonicus exhibited a glycocalyx stained with ruthenium red. Their plasma membrane possessed receptors for Con A, which showed different distributions because of the cell type. All these hemocyte types have been identified in light microscopy and the evolution of hemogram studied in an intermolt.