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USE OF THE DYE HOECHST 33258 IN A MODIFICATION OF THE BROMODEOXYURIDINE PHOTOLYSIS TECHNIQUE FOR THE ANALYSIS OF DNA REPAIR

1980; Wiley; Volume: 31; Issue: 3 Linguagem: Inglês

10.1111/j.1751-1097.1980.tb03710.x

1751-1097

Barry S. Rosenstein, R. B. Setlow, Farid Ahmed,

DNA and Nucleic Acid Chemistry

Abstract— A modification of the bromodeoxyuridine photolysis technique is described in which the cells are treated with the fluorescent dye Hoechst 33258 to enhance the bromodeoxyuridine photolysis produced by 365 nm or by black light. Use of the modified assay overcomes several difficulties encountered in the original 313 nm photolysis technique and demonstrates that excision repair of damage produced in the DNA by 7,12‐dimethylbenz(fl)anthracene 5,6‐oxide takes place by a long patch type of repair pathway.