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Detection of Chromosome 6 Abnormalities in Melanoma Cell Lines by Chromosome Arm Painting Probes

1998; Elsevier BV; Volume: 107; Issue: 2 Linguagem: Inglês

10.1016/s0165-4608(98)00080-6

1873-4456

Xin‐Yuan Guan, Hongen Zhang, Jinming Yang, Jane Wang, Raymond Taetle, Paul S. Meltzer, Jeffrey M. Trent,

Cancer Genomics and Diagnostics

Chromosome 6 abnormalities, particularly the deletion of 6q, are among the most frequent chromosomal changes in human malignant melanoma. In this study, chromosome 6 rearrangements in 21 melanoma cell lines were identified by fluorescence in situ hybridization (FISH) with the use of chromosome 6 arm specific painting probes (CAPs). Structural abnormalities of chromosome 6 were detectable in 18 of 21 (86%) cases, including the identification of structural abnormalities that were not detectable by routine G-banding analysis. The results of this study correlate the frequency of 6q alterations and demonstrate that FISH with CAPs can be of considerable use for rapid screening of specific chromosome abnormalities in melanoma.