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BIBTEX RIS

Amino acid composition of proteins eluted from polyacrylamide gels: Background considerations

1980; Elsevier BV; Volume: 101; Issue: 2 Linguagem: Inglês

10.1016/0003-2697(80)90189-x

ISSN

1096-0309

Autores

William E. Brown, Gary C. Howard,

Tópico(s)

Mass Spectrometry Techniques and Applications

Resumo

It is shown that the contribution of the gel itself to amino acid compositions of proteins eluted from polyacrylamide gels is significant (especially at low protein-to-gel ratios), linearly related to the volume of the gel slices eluted, and, when applied as a correction to amino acid composition data, results in an enhancement of the composition determination.

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