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Effect of seed lectins from Phaseolus vulgaris on the development of larvae of Callosobruchus maculatus ; mechanism of toxicity

1984; Wiley; Volume: 35; Issue: 4 Linguagem: Inglês

10.1002/jsfa.2740350402

1097-0010

Angharad M. R. Gatehouse, Frances M. Dewey, John A. Dove, Katherine A. Fenton, Árpád Pusztai,

Entomopathogenic Microorganisms in Pest Control

Abstract Seeds of the kidney bean ( Phaseolus vulgaris ) are toxic to developing larvae of the bruchid beetle ( Callosobruchus maculatus ), a major storage pest of many legumes. Insect feeding trials were carried out whereby the albumin and globulin protein fractions from seeds of P. vulgaris were incorporated into artificial seeds. Both fractions were shown to be toxic and to contain haemagglutinating activity, implicating the seed lectins as being involved in seed resistance. Further feeding trials using different P. vulgaris lectin preparations confirmed the toxicity of these lectins and suggested that it was the E‐type lectin subunits (erythrocyte‐binding) which were the major antimetabolites. Indirect immunofluorescence investigations using monospecific antisera for globulin lectins showed that the lectins, when ingested by the larvae, bound to the midgut epithelial cells. It was suggested that the mechanism of lectin toxicity in this instance is analogous to that known to occur in the rat, namely that the ingested lectin causes disruption of the epithelial cells of the larval midgut leading to breakdown of the transport of nutrients into these cells, and the absorption of potentially harmful substances. This is the first time that evidence for the mechanism of lectin toxicity has been obtained in insects.