Produção Nacional
Revisado por pares
Aspirin and indomethacin reduce lung inflammation of mice exposed to cigarette smoke
2009; Elsevier BV; Volume: 77; Issue: 6 Linguagem: Inglês
10.1016/j.bcp.2008.12.012
ISSN1873-2968
AutoresP.M.S.T. de Castro, Helena Nasser, Agessandro Abrahão, Larissa Cardilo dos Reis, Ingred Riça, Samuel Santos Valença, Daniele C. Rezende, Luis Eduardo M. Quintas, Moisés C.M. Cavalcante, Luís Cristóvão Pôrto, Vera Lúcia Gonçalves Koatz,
Tópico(s)Inflammatory mediators and NSAID effects
ResumoNeutrophil accumulation response to cigarette smoke (CS) in humans and animal models is believed to play an important role in pathogenesis of many tobacco-related lung diseases. Here we evaluated the lung anti-inflammatory effect of aspirin and indomethacin in mice exposed to CS. C57BL/6 mice were exposed to four cigarettes per day during 4 days and were treated i.p. with aspirin or indomethacin, administered each day 1 h before CS exposure. Twenty four hours after the last exposure, cells and inflammatory mediators were assessed in bronchoalveolar lavage (BAL) fluid and the lungs used for evaluation of lipid peroxidation, p38 mitogen-activated protein kinase (MAPK) phosphorylation and nuclear transcription factor κB (NF-κB) activation. Exposure to CS resulted in a marked lung neutrophilia. Moreover, the levels of oxidative stress-related lipid peroxidation, prostaglandin E2 (PGE2), interleukin 1β (IL-1β), monocyte chemotactic protein 1 (MCP-1), and activated NF-κB and p38 MAPK were greatly increased in CS group. Aspirin or indomethacin treatment led to a significant reduction of neutrophil influx, but only aspirin resulted in dramatic decrease of inflammatory mediators. Moreover, both drugs reduced lung p38 MAPK and NF-κB activation induced by CS. These results demonstrate that short-term CS exposure has profound airway inflammatory effects counteracted by the anti-inflammatory agents aspirin and indomethacin, probably through COX-dependent and -independent mechanisms.
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